Transcription is a fundamental process that must be precisely regulated for cells to function and for organisms to develop. Pioneer transcription factors (PTFs) play a special role by opening chromatin and activating gene expression programmes that define cell identity. Recent studies show that many PTFs form liquid-like condensates, which are key to regulating this process. Our group (www.wittmannlab.com) investigates how such condensates form, what proteins they contain, and how they influence chromatin. Using recombinant proteins and advanced single-molecule assays, we reconstitute these processes in vitro to uncover the molecular principles of transcriptional programming.

This project explores how PTFs use biomolecular condensation to remodel chromatin structure. While PTFs are known to access DNA within compacted chromatin, the role of their condensates in facilitating chromatin opening remains unclear. To address this, chromatin is reconstituted in vitro using fluorescently labeled histones and defined DNA templates, and its interaction with PTFs is studied using single-molecule techniques. These include optical tweezers to detect nucleosome unwrapping through force spectroscopy, and the carpet assay to visualize condensate formation on surface immobilized chromatin via TIRF microscopy. Together, these approaches allow to dissect how PTF condensation influences chromatin accessibility at the single-molecule level.

Experience in protein purification and fluorescence microscopy is of advantage but not mandatory, you will receive training in the respective techniques in our laboratory. Besides standard molecular biology techniques (SDS-PAGE, molecular cloning, etc.) you will apply some of the following specialized methods during the course of the proposed project:

• Excitement about Condensation and/or Chromatin Biology and Single-Molecule Imaging

• Very good oral and written communication skills in English

As a master student at IMB, you will be part of our Internship Programme (www.imb.de/internships). We offer a stimulating, diverse and international research environment, with a pleasant working atmosphereand the opportunity to perform state-of-the-art experiments. The institute is modern, well-equipped andcentrally located with good public transport links and parking.

• Advanced training opportunities

• Team events

• Flexible working hours

• Starting Date: flexible, between October 2025 and January 2026

• Duration: 6 months

• Deadline: 30.09.2025

To apply, please upload your application containing your cover letter, CV, certificates and contact information of at least one professional references to our application form on imb.de/jobs/scientificpositions. Should you have any questions regarding the application process, please contact us by email hr@imb-mainz.de, 
quoting Ref. No. #SWIMS01. Informal enquires should be addressed to Dr Sina Wittmann. 
IMB is an equal opportunity employer.